tag:blogger.com,1999:blog-36768584.post8480387942361506118..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: An Incomplete Guide to Asking for Help on Your De Novo Genome ProjectKeith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger5125tag:blogger.com,1999:blog-36768584.post-6098619893268317502013-07-30T09:33:31.381-04:002013-07-30T09:33:31.381-04:00SMRT works well on very difficult genomes; if your...SMRT works well on very difficult genomes; if your goal is a high quality assembly for any genome less than 20Mb in size, I think it is the clear choice. I would certainly consider it for much larger, but the costs vs. ILMN start to get ugly.Keith Robisonhttps://www.blogger.com/profile/04765318239070312590noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-9528879881944355272013-07-28T00:54:14.928-04:002013-07-28T00:54:14.928-04:00Excellent post. What do you think about PacBio'...Excellent post. What do you think about PacBio's hierarchical genome-assembly process? They claim to achieve accurate assembly of microbial species using data from just one long read SMRT shotgun library.Genohubhttp://www.genohub.comnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-68616258482411122822013-05-19T05:45:39.165-04:002013-05-19T05:45:39.165-04:00Nice consolidation - another good resource is mail...Nice consolidation - another good resource is mailing lists of assembly programs such as Mira etc. If not anything, at least you find that there are many that share your pain.Nandita Mullapudinoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-20035738181781341062013-05-17T13:19:35.142-04:002013-05-17T13:19:35.142-04:00Shawn: thanks for catching that! Fixed.Shawn: thanks for catching that! Fixed.Keith Robisonhttps://www.blogger.com/profile/04765318239070312590noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-6423926777519425222013-05-17T13:05:35.676-04:002013-05-17T13:05:35.676-04:00Great stuff! But you should fix the following typo...Great stuff! But you should fix the following typo to prevent confusing future readers:<br /><br />"At this moment in time (May <b>2012</b>)..."Anonymoushttps://www.blogger.com/profile/08590569202982138251noreply@blogger.com