tag:blogger.com,1999:blog-36768584.post8125120968665352882..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: PacBio Revio: Same Footprint, 80% The Time, 15X The HiFi! Keith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger5125tag:blogger.com,1999:blog-36768584.post-19314857299559645052022-12-10T14:01:37.039-05:002022-12-10T14:01:37.039-05:00For anyone interested in exploring Revio data, Pac...For anyone interested in exploring Revio data, PacBio is releasing five human datasets generated from Genome In A Bottle samples. <br /><br />deepika kapoorhttps://www.blogger.com/profile/00139617591378474245noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-62816449707807752432022-10-26T16:21:37.617-04:002022-10-26T16:21:37.617-04:00John: The 25M is a the number of features on the d...John: The 25M is a the number of features on the device; loading efficiencies are typically in the 33% range and then another fraction of the loaded ZMWs will not generate a HiFi read<br /><br />So probably 25% of the ZMW count will actually deliver HiFi reads, which squares the numbers (roughly, of course)Keith Robisonhttps://www.blogger.com/profile/04765318239070312590noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-84620027249582839302022-10-26T15:57:38.467-04:002022-10-26T15:57:38.467-04:00Hi Keith - thanks for the nice summary. Can you el...Hi Keith - thanks for the nice summary. Can you elaborate on the throughput? A 30x human genome per flowcell is ~90GB. At 25M ZMW per flowcell this works out to an average of 3.6 Kb reads. But they are claiming read sizes of 15-18 kb. What am I missing?John Didionnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-69433699170761671342022-10-26T15:54:35.436-04:002022-10-26T15:54:35.436-04:00Both have their merits. I see ONT and PacBio being...Both have their merits. I see ONT and PacBio being different tools for different problems. Illumina is the cost effective solution where short reads will suffice (most resquencing applications). ONT is great where you need quick, cheap, and very long reads but base calling quality is not as important. PacBio seems to be positioning itself somewhere in between for cases where you need longer reads than Illumina while maintaining high accuracy.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-42567894586198106392022-10-26T11:42:20.129-04:002022-10-26T11:42:20.129-04:00significant upgrade but still less data and more c...significant upgrade but still less data and more cost than an ONT P24.Anonymousnoreply@blogger.com