tag:blogger.com,1999:blog-36768584.post7713957622029382772..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: What will be the last Sanger genome?Keith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-36768584.post-76400550490206498702011-04-06T01:57:35.957-04:002011-04-06T01:57:35.957-04:00Sanger will still be with us for quite a while - t...Sanger will still be with us for quite a while - there will always be a need for real reference genomes that can't be bootstrapped with short reads. Will you know when the last one is sequenced? Probably not, as Sanger has taken on a kind of support role for complex genomes where it provides the backbone which is filled in with 454 or illumina reads.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-85662351399285241112011-03-09T16:30:44.185-05:002011-03-09T16:30:44.185-05:00I have to agree, in the last month I have been mee...I have to agree, in the last month I have been meeting sequencing centers and molecular diagnostic lab. They give me a weird look when we start talking about GC challenges, homopolymer read through and error rates. Designing amplicon enrichment strategies over short strand is not always simple in highly mutated genomes such as viruses. I can see a lot of labs are going to realise that they are gonna have to clean things out with Sanger. Unless that super dupper Pac Bio dream machine junior with 0,1% error rate at 50K comes out...Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-32223770548166723872011-03-09T13:28:54.570-05:002011-03-09T13:28:54.570-05:00For complete genome sequencing, indeed, CE/Sanger ...For complete genome sequencing, indeed, CE/Sanger sequencing is a poor choice. But for every genome sequenced there are many hundreds of hypotheses generated and they need to be followed up with accurate, long-read, single template sequencing. There is still no better technology out there for that. CE/Sanger sequencing will increase rather than decrease, at least for the next several years.Unknownhttps://www.blogger.com/profile/09278530960257085769noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-88330527506080029962011-03-08T13:05:23.035-05:002011-03-08T13:05:23.035-05:00I think that 454 sequencing will still be favored ...I think that 454 sequencing will still be favored for bacterial and archaeal genomes for some time. THe long reads make for much easier assembly than Illumina or SOLiD reads, and for de novo short genomes, the analysis cost exceeds the sequencing cost by a lot.Anonymousnoreply@blogger.com