tag:blogger.com,1999:blog-36768584.post4419723931916779426..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: Can BGI Really Stir Up the Sequencing Instrument Market?Keith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger8125tag:blogger.com,1999:blog-36768584.post-6119081267103992432015-06-16T14:56:31.141-04:002015-06-16T14:56:31.141-04:00See here for info about the new BGI/CG sequencing ...See here for info about the new BGI/CG sequencing platform: http://bgi-international.com/services/genomics/platforms/revolocity/Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-6459324059527676842015-03-06T10:19:41.647-05:002015-03-06T10:19:41.647-05:00It's interesting to consider how in situ seque...It's interesting to consider how in situ sequencing will play out in this context. With in situ sequencing we can already fill the cellular space completely with rolonies (which are approximately diffraction limited in size), such that our imaging is very efficient (~4-8 pixels per rolony, with no "dark" space). Since our reads are single molecule by nature, no throughput is wasted sequencing PCR clones. Because we image in 3D, the sequencing time is very efficient (we can sequence an Illumina-sized flow cell in XY dimensions, but also 25 or 50 um in Z simultaneously). It is also possible to combine RNA-seq with genomic sequencing simultaneously, such that over a moderate number of cells one could achieve genomic coverage, while also getting thousands of RNA-seq data-points per cell. Spatial information in sequencing is an interesting new frontier.<br /><br />By the way I'm a long-time reader of your blog, but first time commenting. I'm one of the first authors on the in situ sequencing paper published in Science last year from George Church's lab.Evan Daugharthynoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-49087474463809973062015-03-05T22:23:09.073-05:002015-03-05T22:23:09.073-05:00Didn't Illumina just claim their NextSeq V2 ch...Didn't Illumina just claim their NextSeq V2 chemistry is as good as HiSeq? If that's true and the next iteration of HiSeq X uses 2-color chemistry, the throughput can at least double. So no worry for Illumina for now. Geneticist from the Eastnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-6527633794763025152015-03-05T17:43:51.676-05:002015-03-05T17:43:51.676-05:00On some level it seems kind of preposterous that B...On some level it seems kind of preposterous that BGI/CG can come up with something that would immediately wipe the floor versus Illumina sequencers. Aside from breaking the laws of physics regarding imaging speed and quality, it is really telling that there have been little reports in the media and scientific crowds about the technology. Unless the culture of BGI is to be incredibly secretive -- moreso than that of Apple -- the lack of news is not a great sign.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-36624058074312636932015-03-05T10:04:21.320-05:002015-03-05T10:04:21.320-05:00AT the VIB conference in February, the CSO of Comp...AT the VIB conference in February, the CSO of Complete Genomics presnted on these and mentioned two key things:<br />1) Their LFR (Long Fragment Read) methods, using 10kb-1Mb - published in Frotiers in Genetics http://journal.frontiersin.org/article/10.3389/fgene.2014.00466/abstract<br />2) the claim they can sequence 18 genomes per run - 60 billion reads/3Tb per run (98% exome bases called at error rate of 1/Mb)with target of $1/Gb.<br /><br />Look forward to seeing what happens....LMikeFnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-65065596962230326492015-03-05T07:51:58.288-05:002015-03-05T07:51:58.288-05:00Talk is cheap...I'm from Missouri.Talk is cheap...I'm from Missouri.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-49702276670308453572015-03-05T04:08:28.048-05:002015-03-05T04:08:28.048-05:00i don't beleive they will sell the instrument ...i don't beleive they will sell the instrument around the world, they don't have a large group of sale, engineer to support it. There is a huge need for the chinese market for using sequencer so they will just become dominated in the service industry, but it is very excited to see another technology!xiaonanoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-42464258964658164562015-03-04T23:31:00.954-05:002015-03-04T23:31:00.954-05:00a $1 genome in 5 years? Right.
I do hope they ha...a $1 genome in 5 years? Right.<br /><br />I do hope they have something decent though. It might make dealing with Illumina more bearable. I'm not sure how many of us would be interested in anything shorter than 100bp. That seems more of a step back than a step forward.Brian Kruegerhttps://www.blogger.com/profile/13456672262242780223noreply@blogger.com