tag:blogger.com,1999:blog-36768584.post1213603238386816503..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: Post-AGBT: MiroculusKeith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-36768584.post-45442473538909153042020-03-14T13:55:36.881-04:002020-03-14T13:55:36.881-04:00Dr. Robison:
It's now all corona all the time...Dr. Robison:<br /><br />It's now all corona all the time; you are not keeping pace! I had thought this wasn't going to affect me -- now there's no toilet paper in our store --; even the food sections are becoming barren. People are worried.<br /><br />I am sure that you are very busy, though if you have a chance might you write a column that considered die Gedankenerfahrung in which everyone had a nanopore sequencer at home. Nanosequencer as human right. <br /><br />Waiting for highly centralized medical systems to respond clearly can be seen to be an inefficient strategy. The natural tendency is to conceal the truth, then deny the truth, and ultimately to be overwhelmed by the truth. <br /><br />Moving pathogen surveillance to the level of the individual would help avoid such counter-productive behavior. At what point would a sequencer identify a coronavirus infection? (i.e., would this be before someone became infectious?). In such a idealized world, would a viral pandemic even be possible? (If people could rapidly self-diagnose, then wouldn't this rapidly halt any such epidemic before it became a pandemic?). How economically and technically feasible would it be as of now for DIY<br />viral nanosequencing? For a diagnosis one would not need the complete viral sequence, only definitive DNA reads. Would a Flonge be enough? <br /><br /> <br />Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-29375811292720901442020-03-13T16:41:48.565-04:002020-03-13T16:41:48.565-04:00Adam from Miroculus here. We have done a lot of w...Adam from Miroculus here. We have done a lot of work to understand any trace material left behind by different reagents. We generally do not see any contamination in zones where reagents have tracked, with the exception of some biofouling in the thermalcycling zones. The software automatically avoids these zones after thermalcycling is performed. The cartridges are single use, so we don't have to worry about sample-to-sample carryover. Hope that helps!Anonymoushttps://www.blogger.com/profile/05269205266138619073noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-53731140223584627062020-03-12T17:20:56.971-04:002020-03-12T17:20:56.971-04:00I'm wondering how vulnerable a system like thi...I'm wondering how vulnerable a system like this is to cross-contamination (due to residual reagent sticking to a given spot and then mixing with a reagent that is subsequently moved to that spot). Are washing routines required, such that a washing solution 'follows' each droplet of reagent? Or, is the transport so clean that this isn't needed. And, are certain buffers problematic? In particular for fouling? I'm excited about these library prep systems and seeing what other areas of molecular biology they can be used for, but I'd like to get a sense for their limitations.Anonymousnoreply@blogger.com