tag:blogger.com,1999:blog-36768584.post9182146484383837394..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: My Most Expensive PaperKeith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger8125tag:blogger.com,1999:blog-36768584.post-11609146104581287982009-11-27T09:04:34.521-05:002009-11-27T09:04:34.521-05:00My strategy these days is to PCR from a polyA cDNA...My strategy these days is to PCR from a polyA cDNA prep and if I don't get it (usually anything over 1000bp) I order something. I was considering buying the FANTOM3 collection recently ($30,000 for 100,000 cDNAs vs the same amount for 50 ORFs) but really like you say if synthesis gets cheaper every year it's difficult to justify the investment. The individual clones will likely get cheaper too.Anthonynoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-5037448554297684672009-11-24T22:20:22.710-05:002009-11-24T22:20:22.710-05:00Well, you've hit the crux of the question. MG...Well, you've hit the crux of the question. MGC has cloned or built all these genes so you can get them essentially overnight -- but a lot of money went into building them & there is expense to maintaining the collection. <br /><br />Alternatively, you can try to clone things yourself or have them built, but that involves some uncertainty of success and a delay in your project. Indeed, the whole reason we were building clones is that these had failed to be PCR cloned in multiple attempts.<br /><br />So, is it worth investing in having a ready-to-go bank of mammalian ORFs, or is it really better to pay-as-you go, especially if you think that synthetic biology will get continually cheaper.Keith Robisonhttps://www.blogger.com/profile/04765318239070312590noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-1256352360976523902009-11-23T09:49:17.179-05:002009-11-23T09:49:17.179-05:00P.S. Correction. $700 is the price for ORFome clon...P.S. Correction. $700 is the price for ORFome clones which is not necessarily the same as MGC clones (but probably the same when synthesized).Anthonynoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-46801363510228261822009-11-23T09:32:30.195-05:002009-11-23T09:32:30.195-05:00Thanks. I was just wondering if everyone would hav...Thanks. I was just wondering if everyone would have been better off if Codon Devices had been selling clones to scientists directly. Currently MGC clones go for ~$700 each. Cheaper to order a full length cDNA and PCR clone it. Buying a few is OK but in large numbers not really justifiable.Anthonynoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-51058862789217972312009-11-22T17:38:48.003-05:002009-11-22T17:38:48.003-05:00I'm not sure what we actually charged for the ...I'm not sure what we actually charged for the MGC project, but in those days we tended to quote around $0.75/bp for short, easy stuff and $1.50-$2.00 for the long, hard stuff. Most of MGC would have been the latter.<br /><br />The short, easy category probably averaged 1.5Kb, so perhaps just over $1K/gene. The long, hard stuff had a lot of 3-5Kb stuff (and some longer), so a lot of genes at as much as $10K each.<br /><br />If you Google for "gene synthesis", some places are as low as $0.35/bp -- but I'll bet that's only for really easy (i.e. codon optimized) sequences. Without getting actual quotes, it's hard to know what you'll get charged -- and until it shows up you don't know when (or if) it does. Most things will show up, but it's still tricky to figure out how hard it will be to make something.Keith Robisonhttps://www.blogger.com/profile/04765318239070312590noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-10444204786594302592009-11-22T13:53:32.704-05:002009-11-22T13:53:32.704-05:00Just curious. What was the budget for this project...Just curious. What was the budget for this project? Would be interesting to know how much the easy first 1000 ORFs cost vs the difficult last 1000.Anthonynoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-42366754610360418532009-11-21T02:14:54.888-05:002009-11-21T02:14:54.888-05:00This comment has been removed by a blog administrator.Paper Researchhttp://www.researchpaperspot.com/noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-75421887534140666602009-10-30T00:07:27.611-04:002009-10-30T00:07:27.611-04:00Sounds unfortunately like the discussions I rememb...Sounds unfortunately like the discussions I remember from undergrad about whether it made more sense to go straight to grad school or work as a tech. <br /><br />Doing the exciting work is way more fun than doing the grunt work to enable other people to do exciting research. <br /><br />Hope your present employment lets you spend more time doing breakthrough stuff yourself.Jameshttp://www.jamesandthegiantcorn.comnoreply@blogger.com