tag:blogger.com,1999:blog-36768584.post6508421969309106890..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: New fangled DNA sequencingKeith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-36768584.post-9924368306168158872007-02-09T22:36:00.000-05:002007-02-09T22:36:00.000-05:00Sigh. The risk of working on a blog with too litt...Sigh. The risk of working on a blog with too little sleep and too much deadline pressure.Keith Robisonhttps://www.blogger.com/profile/04765318239070312590noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-64612601170590238122007-02-09T07:52:00.000-05:002007-02-09T07:52:00.000-05:00Thanks for the plug, but I'm not PZ Myers :)I'm ho...Thanks for the plug, but I'm not PZ Myers :)<BR/><BR/>I'm hoping that 454 and similar technologies will revolutionize de novo sequencing in eukaryotes, but the issue of paired end reads will cause problems. Also, the short read lengths will be troublesome for repetitive eukaryotic genomes.<BR/><BR/>I think 454 could be useful for resequencing in eukaryotes, but the accuracy of 454 reads may interfere with the purpose of resequencing projects (accurate detection of polymorphism). For example, 454 runs into problems with mononucleotide repeats.RPMhttps://www.blogger.com/profile/00344508931818143159noreply@blogger.com