tag:blogger.com,1999:blog-36768584.post4477565381583948694..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: Genia Publishes Platform ProgressKeith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-36768584.post-72181151167277686662016-05-23T15:45:43.627-04:002016-05-23T15:45:43.627-04:00Keith, I'd be careful in comparing this to the...Keith, I'd be careful in comparing this to the "Genia" sequencer. This is work done at Columbia with Genia's support. Genia licensed the Columbia patent and this work was done on Columbia's proof of concept system. What the actual Genia sequencer is capable of given the time they've sat with Roche's money and the Columbia patent is anyone's guess.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-50429844872014483842016-04-29T04:04:05.853-04:002016-04-29T04:04:05.853-04:00You're write up is characteristically better t...You're write up is characteristically better than mine. :) I thought the data shown in figure 5 was quite nice, the data seems very clean, much more so than the convolved signal you get from strand systems. So that's nice, and gives some hope that the system could provide better data quality. But this is a really basic proof of concept, no doubt many other issues before it could be productionized. The take home message for me was "this basic approach works, but it's too early to say anything about data quality, however it has single base resolution which is nice".<br /><br />Figure 6 uses a different set of conditions to get round stuttering, I also didn't find this very convincing, particularly without access to the raw data (grumble). They're obviously isn't much "scale" in this work as they say they are manually base calling everything.<br /><br />One other statement I didn't understand was this: "The applied voltage is adjusted to ensure that, in a majority of cases, one and only one pore is inserted into the membranes of each well." which seems to imply that they have a way of breaking the poisson limit associated with other nanopore systems and stopping more than one pore inserting. I didn't think this was a known/common technique, so I'd be interested in literature on that.<br /><br />Anyway, overall I thought it was interesting incremental progress. The system seems somewhat complex compared to other nanopore systems, and I would have thought that would make building a commercial platform challenging, but who knows? I'd guess a lot of it depends on the execution.newhttps://www.blogger.com/profile/16659709753088495544noreply@blogger.com