tag:blogger.com,1999:blog-36768584.post1307109333565236319..comments2024-03-03T18:49:34.382-05:00Comments on Omics! Omics!: PacBio Back of the Envelope NumbersKeith Robisonhttp://www.blogger.com/profile/04765318239070312590noreply@blogger.comBlogger5125tag:blogger.com,1999:blog-36768584.post-86907467773524630972013-10-07T08:17:45.143-04:002013-10-07T08:17:45.143-04:00I agree, PacBio is actually conservative on what i...I agree, PacBio is actually conservative on what is achievable on their chemistries. However, the variance can be quite large between samples due to differences in insert length or getting the loading concentration right, so there is good reason to not rely getting monster runs every time.Keith Robisonhttps://www.blogger.com/profile/04765318239070312590noreply@blogger.comtag:blogger.com,1999:blog-36768584.post-17983870839065457582013-10-07T01:58:28.615-04:002013-10-07T01:58:28.615-04:00Seems like official stats are often less than what...Seems like official stats are often less than what people yield in practice. e.g. Duke tweet with 658 MB from a single cell with P4/c2. So even though the official throughput of the new P5/c3 chemistry - with N50 read length of 10kb - is only 350 MB per cell I would expect actual yields to be higher. As high as 1 GB per cell even? Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-46955382146670447812013-03-27T07:12:55.833-04:002013-03-27T07:12:55.833-04:00Masz dobre zdanie na różne tematy.Masz dobre zdanie na różne tematy.Wynajem kasyna warszawahttp://akademiaimprez.plnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-69160440237449353482013-03-06T19:22:23.498-05:002013-03-06T19:22:23.498-05:00Nice analysis. Your price range for a PacBio instr...Nice analysis. Your price range for a PacBio instrument is a bit low, if anything. Your data also explains why PACB has no real path towards profitability without a massive increase in the installed base, which just seems implausible. RS is a nice tool and can be used alone or paired with ILMN for certain applications, but will never find much of a home outside of large academic sequencing centers.bioduediligencehttp://www.biotechduediligence.comnoreply@blogger.comtag:blogger.com,1999:blog-36768584.post-70160583518421352022013-03-04T02:50:54.271-05:002013-03-04T02:50:54.271-05:00Nice post! You may have seen I am thinking along t...Nice post! You may have seen I am thinking along the same lines: http://flxlexblog.wordpress.com/2013/02/11/applications-for-pacbio-circular-consensus-sequencing/Anonymousnoreply@blogger.com